Single View Reconstruction for Human Face and Motion with Priors

Single view reconstruction is fundamentally an under-constrained problem. We aim to develop new approaches to model human face and motion with model priors that restrict the space of possible solutions. First, we develop a novel approach to recover the 3D shape from a single view image under challenging conditions, such as large variations in illumination and pose. The problem is addressed by employing the techniques of non-linear manifold embedding and alignment. Specifically, the local image models for each patch of facial images and the local surface models for each patch of 3D shape are learned using a non-linear dimensionality reduction technique, and the correspondences between these local models are then learned by a manifold alignment method. Local models successfully remove the dependency of large training databases for human face modeling. By combining the local shapes, the global shape of a face can be reconstructed directly from a single linear system of equations via least square. Unfortunately, this learning-based approach cannot be successfully applied to the problem of human motion modeling due to the internal and external variations in single view video-based marker-less motion capture. Therefore, we introduce a new model-based approach for capturing human motion using a stream of depth images from a single depth sensor. While a depth sensor provides metric 3D information, using a single sensor, instead of a camera array, results in a view-dependent and incomplete measurement of object motion. We develop a novel two-stage template fitting algorithm that is invariant to subject size and view-point variations, and robust to occlusions. Starting from a known pose, our algorithm first estimates a body configuration through temporal registration, which is used to search the template motion database for a best match. The best match body configuration as well as its corresponding surface mesh model are deformed to fit the input depth map, filling in the part that is occluded from the input and compensating for differences in pose and body-size between the input image and the template. Our approach does not require any makers, user-interaction, or appearance-based tracking. Experiments show that our approaches can achieve good modeling results for human face and motion, and are capable of dealing with variety of challenges in single view reconstruction, e.g., occlusion

A novel link between the proteasome pathway and the signal transduction pathway of the Bone Morphogenetic Proteins (BMPs)

BACKGROUND: The intracellular signaling events of the Bone Morphogenetic Proteins (BMPs) involve the R-Smad family members Smad1, Smad5, Smad8 and the Co-Smad, Smad4. Smads are currently considered to be DNA-binding transcriptional modulators and shown to recruit the master transcriptional co-activator CBP/p300 for transcriptional activation. SNIP1 is a recently discovered novel repressor of CBP/p300. Currently, the detailed molecular mechanisms that allow R-Smads and Co-Smad to co-operatively modulate transcription events are not fully understood. RESULTS: Here we report a novel physical and functional link between Smad1 and the 26S proteasome that contributes to Smad1- and Smad4-mediated transcriptional regulation. Smad1 forms a complex with a proteasome β subunit HsN3 and the ornithine decarboxylase antizyme (Az). The interaction is enhanced upon BMP type I receptor activation and occur prior to the incorporation of HsN3 into the mature 20S proteasome. Furthermore, BMPs trigger the translocation of Smad1, HsN3 and Az into the nucleus, where the novel CBP/p300 repressor protein SNIP1 is further recruited to Smad1/HsN3/Az complex and degraded in a Smad1-, Smad4- and Az-dependent fashion. The degradation of the CBP/p300 repressor SNIP1 is likely an essential step for Smad1-, Smad4-mediated transcriptional activation, since increased SNIP1 expression inhibits BMP-induced gene responses. CONCLUSIONS: Our studies thus add two additional important functional partners of Smad1 into the signaling web of BMPs and also suggest a novel mechanism for Smad1 and Smad4 to co-modulate transcription via regulating proteasomal degradation of CBP/p300 repressor SNIP1

Conformational Toggling of Yeast Iso-1-Cytochrome c in the Oxidized and Reduced States

To convert cyt c into a peroxidase-like metalloenzyme, the P71H mutant was designed to introduce a distal histidine. Unexpectedly, its peroxidase activity was found even lower than that of the native, and that the axial ligation of heme iron was changed to His71/His18 in the oxidized state, while to Met80/His18 in the reduced state, characterized by UV-visible, circular dichroism, and resonance Raman spectroscopy. To further probe the functional importance of Pro71 in oxidation state dependent conformational changes occurred in cyt c, the solution structures of P71H mutant in both oxidation states were determined. The structures indicate that the half molecule of cyt c (aa 50–102) presents a kind of “zigzag riveting ruler” structure, residues at certain positions of this region such as Pro71, Lys73 can move a big distance by altering the tertiary structure while maintaining the secondary structures. This finding provides a molecular insight into conformational toggling in different oxidation states of cyt c that is principle significance to its biological functions in electron transfer and apoptosis. Structural analysis also reveals that Pro71 functions as a key hydrophobic patch in the folding of the polypeptide of the region (aa 50–102), to prevent heme pocket from the solvent

Accurate 3D Pose Estimation From a Single Depth Image

This paper presents a novel system to estimate body pose configuration from a single depth map. It combines both pose detection and pose refinement. The input depth map is matched with a set of pre-captured motion exemplars to generate a body configuration estimation, as well as semantic labeling of the input point cloud. The initial estimation is then refined by directly fitting the body configuration with the observation (e.g., the input depth). In addition to the new system architecture, our other contributions include modifying a point cloud smoothing technique to deal with very noisy input depth maps, a point cloud alignment and pose search algorithm that is view-independent and efficient. Experiments on a public dataset show that our approach achieves significantly higher accuracy than previous state-of-art methods. 1

Involvement of Bim in Photofrin-Mediated Photodynamically Induced Apoptosis

Background/Aims: Photodynamic therapy (PDT) is a promising noninvasive technique, which has been successfully applied to the treatment of human cancers. Studies have shown that the Bcl-2 family proteins play important roles in PDT-induced apoptosis. However, whether Bcl-2-interacting mediator of cell death (Bim) is involved in photodynamic treatment remains unknown. In this study, we attempt to determine the effect of Bim on Photofrin photodynamic treatment (PPT)-induced apoptosis in human lung adenocarcinoma ASTC-a-1 cells. Methods: The translocation of Bim/Bax of the cells were monitored by laser confocal scanning microscope. The levels of Bim protein and activated caspase-3 in cells were detected by western blot assay. Caspase-3 activities were measured by Caspase-3 Fluorogenic Substrate (Ac-DEVD-AFC) analysis. The induction of apoptosis was detected by Hoechst 33258 and PI staining as well as flow cytometry analysis. The effect of Bim on PPT-induced apoptosis was determined by RNAi. Results: BimL translocated to mitochondria in response to PPT, similar to the downstream pro-apoptotic protein Bax activation. PPT increased the level of Bim and activated caspase-3 in cells and that knockdown of Bim by RNAi significantly protected against caspase-3 activity. PPT-induced apoptosis were suppressed in cells transfected with shRNA-Bim. Conclusion: We demonstrated the involvement of Bim in PPT-induced apoptosis in human ASTC-a-1 lung adenocarcinoma cells and suggested that enhancing Bim activity might be a potential strategy for treating human cancers

Numerical and Experimental Investigation on the Effect of Mechanical Smoke Extraction Caused by External Wind in Subway Station Halls

When fires break out in subway station halls, traditional smoke extraction (TSE) systems are employed with the aim of preventing smoke from spreading to the platform and passageways. The functionality of TSE systems under the influence of external winds needs to be further explored. Based on a numerical method, this study investigated the effect on TSE systems under the influence of external wind. A numerical model was established and validated by means of full-scale field tests to ensure accuracy. Subsequently, the validated model was applied to study the effect of the external wind directions and speeds on the smoke diffusion distance. The results showed that when all entrances and exits were on the windward side, the external wind direction led to serious longitudinal diffusion of the smoke toward the side with fewer entrances and exits of the station hall, and the diffusion distance increased with increasing wind speed. The diffusion distance reached a maximum value of 61.32 m when the outdoor wind was 5 m/s, which was 67.9% higher than that under no wind. When all the entrances and exits were on the leeward side, the external wind had little influence on the degree of smoke spread, with the greatest smoke diffusion distance being only 4.76% longer than that under no wind. When two entrances and exits were on the windward side and the other on the leeward side, the external wind caused smoke to spread to a passageway, and the degree of smoke spread was more unfavorable at higher wind speeds, with the longest diffusion distance being 7.28 m. To prevent smoke from spreading to passageways and to effectively shorten the longitudinal diffusion distance of smoke, an optimized smoke control (OSC) system was proposed, employing center and passageway smoke barriers, which were able to shorten the diffusion distances by 35.45%, 13.64%, and 2.35%. In particular, smoke diffusion did not occur in passageways. This study provides a reference for the fire safety engineering design of subway stations